Whole mount ihc zebra fish book

Zebrafish whole mount ihc of kidney tissue in embryos 72 hpf. W holemount immunohistochemistry ihc is a valuable tool for investigation of protein expression during zebrafish embryonic development. Immunohistochemistry is also an important complement to gene expression, as translation and transcription may not be concurrent. This mixture has the same refractive index as yolk and clears the embryos very well.

The endoderm indirectly influences morphogenetic movements. Zebra fish fins provide insight into bone regeneration. Whatever fixative you have successfully used in ihc fr with your antibody should also be suitable for whole mount. In whole mount ihc, the distribution of an antigen can be mapped rapidly and reliably in the embryos. The zebrafish book a guide for the laboratory use of zebrafish danio brachydanio rerio by monte westerfield, institute of neuroscience, university of oregon. Zebra fish fins provide insight into bone regeneration date. General wholemount immunohistochemistry of zebrafish. For this reason, ihc is an important supplement to confirm ish data, when possible. Professor anthony graham professor of developmental biology molecular neurobiology, medicine kings college, london, uk whole mount staining of zebrafish embryos, now commonly used, requires extra steps to fix and permeabilize to. Danio rerio commonly known as the zebrafish is a tropical fish belonging to the minnow family cyprinidae, commonly kept in aquaria and used for scientific research. Zebrafish sample preparation for whole mount immunohistochemistry.

Use of phospholipase a2 for antigen retrieval in zebrafish. Histological methods protocols zfin community wiki. An activity map of the whole zebrafish brain mo costandi. Zebrafish exhibit spontaneous contractions mediated by gap junctions beginning at before 19. Finally, ihc can provide subcellular and colocalization data that cannot be determined by ish, 14, 15. Although this concentration of pfa is very low, this has to be left on for a long period of time on whole mount samples to allow permeabilization to the center of the sample. Whole mount immunohistochemistry is a valuable tool for the study of spatial and temporal protein expression during zebrafish development. However, there are relatively few zebrafish antibodies available. A simple wholemount staining protocol for bone and or cartilage in adults and larvae clearing and staining for larval fish cartilage and bone chromosomes spreads.