Good practice for those who have never used a syringe too. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then being a 110 dilution of the previous one in the series. A serial dilution is the stepwise dilution of a substance in solution. Most questions you will be asked to answer about serial dilutions are of two types. What does this mean if a solution has a 110 dilution the number represents 1 part of the patient sample added to 9 parts of diluent. So you multiply each successive dilution by the dilution factor. Serial dilutions a serial dilution is any dilution where the concentration decreases by the same quantity in each successive step. Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively. Serial dilutions practice problems refer to the below image. Producing samples with different concentrations in a series is more time efficient than trying to prepare each sample one by one. I made this for my as biology students who dont take chemistry and needed some practice with serial dilutions before jumping straight into a full practical.
Thus, a series of 5, one to ten dilutions equals a one to one hundred thousand dilution. In medicine, these skills are necessary to ensuring proper medicine dosage and treatment. Then 1 ml from flask a is transferred to flask b and then 1 ml from flask b is transferred to flask c. Concentrations and dilutions answer key worksheets kiddy. Spectrophotometry page 7 of 10 single dilutions background stock solutions are constantly diluted in biological lab settings. Dilutions are useful in science when making solutions or growing an acceptable number of bacterial colonies to count. All materials were written for and are currently used. Commonly the dilution aspect at every step is regular, leading to a geometric progression of the awareness in a logarithmic fashion. Dilutions worksheet i revised sunday, march 24, 20 1 revision 1. You will receive your score and answers at the end. Bacterial dilutions and a foolproof way to figure them out look at the. Dilutions part 3 of 4 calculating colony forming unitsml duration. Discovering ecosystems cary institute of ecosystem studies.
Molarity and serial dilutions teacher handout module overview one of the most important skills in science is to calculate dilutions. Dilutions worksheet i revised sunday, march 24, 20 2 revision 1. Serial dilution practice problem set science primer. The second type gives you the number of bacteria per ml in the original. Choose from 8 different sets of serial dilution flashcards on quizlet. Dilutions worksheet socorro independent school district. Jan 10, 2011 a serial dilution is the stepwise dilution of a substance in solution. Serial dilution displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. The following problem sets test your ability to calculate dilution factors and concentration s. Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.
Note that serial dilution is a special case of series of dilutions. Choose from 184 different sets of serial dilutions flashcards on quizlet. Serial dilutions reduce the concentration of a sample in small steps or fractions. Determine the concentration or dilution factor for the flasks and plates. The answers i sure hope you tried to answer the problems before you looked here. Dilutions worksheet w 329 everett community college student support services program 1 if 45 ml of water are added to 250 ml of a 0. Displaying all worksheets related to serial dilution. Serial dilution worksheet bio152 paramedical microbiology 1. Bacterial dilutions and a foolproof way to figure them out look at the dilution scheme below. The power point can be used to reveal instructions to the students during the lab. It is critical that you master both serial and simple single dilutions. This quiz and attached worksheet will help gauge your understanding of the properties of serial dilution.
Antibiotic concentration from stock solution calculation. The first type gives you the number of bacterial colonies found on a plate and asks for the number of bacteria per ml in the original culture. A serial dilution is a stepwise dilution of a substance in solution. Concentrations and dilutions answer key displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work, dilutions work name key, dilutions work w 329, concentrations and dilutions, molarity and serial dilutions teacher handout, laboratory math ii solutions and dilutions. Sep 21, 2011 a serial dilution is the stepwise dilution of a substance in solution. Utilize the worksheet to identify the more important study. A serial dilution is any dilution where the concentration decreases by the same quantity in each successive step. Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. When we said the diluted coffee was 110th as strong as the original that was a dilution factor. There are three formulas used to work microbiology dilution problems. It is a common practice to determine microbial counts for both liquid and solid specimenssuspensions of e. Serial dilution worksheet bio152 paramedical micro. Students complete prelab and postlab questions and data table.
If one ml was spread on plate a it would be x 10 4 or 000 or 1. Some of the worksheets for this concept are dilutions work, dilutions work, dilutions work w 329, dilutions work name key, making dilutions work, solutions work 2 molarity and dilution problems answers, dilution name chem work 15 5, dilution work answers. Serial dilutions practice problems san diego miramar college. This is called a dilution series or a serial dilution. From the original culture 1 ml of broth was transferred to flask a which contains 99 ml of sterile water. Upon completion of this lab, the student should be able to proficiently calculate molarities for solutions.
Bacterial dilutions and a foolproof way to figure them out. Dilutions worksheet solutions 1 if i have 340 ml of a 0. This worksheet defines stock solution, serial dilution and provides 3 practice problems. Mi x v, mol useful kquations mi x v, m2 x v2 molarity molsolutc l. Learn serial dilutions with free interactive flashcards. Dilutions worksheet solutions 1 if i add 25 ml of water to 125 ml of a 0. Test your knowledge of the properties of serial dilution by using this interactive quiz. Showing top 8 worksheets in the category serial dilution.
Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. Free gamified quizzes on every subject that students play in class and at home. This general microbiology practical lecture explains the serial dilution techniques in pour plate method to isolate bacteria. In a serial dilution, the final total dilution is a product of each individual dilution in the series. A series of five dilutions is made of this first dilution by diluting it 110, rediluting 110, and then three times more, each resulting solution then. Worksheets are dilutions work, dilutions work w 329, dilution name chem work 15 5, dilutions work, dilution work answers, chemistry dilution practice, dilutions work name key, solutions work 2 molarity and dilution problems answers.
In the lab, correct dilutions of solutions are critical to performing accurate science. The following formula can easily be used to carry out a simple dilution. Some of the worksheets displayed are dilutions work, dilutions work, dilutions work w 329, dilutions work name key, making dilutions work, solutions work 2 molarity and dilution problems answers, dilution name chem work 15 5, dilution work answers. What molarity would the potassium nitrate solution need to be if you were only to need 2. Students perform serial dilution of food coloring experiment with partner, examine concentration levels, and differentiate parts per million ppm and parts per billion ppb.
We worked through the first example together on the board and the students did the dilution using coloured water. When youre thinking about dilution, it helps to simplify your actions into dilution factors. Use the following example of a series of dilutions and plating to answer the problems below. We could also have said the dilution factor was 110, or the dilution factor was 0. Worksheets are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. A set of serial dilutions is made, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. To determine the concentration you multiply the number of colonies by the inverse of the dilution factor in flask b it was 104. You multiply the original concentration by the dilution factors for each dilution. Answers serial dilutions practice worksheet biol 307 studocu. When a solution of glucose, c 6h 12o 6, is diluted, the number of moles of the solute in the original solution is greater than, less than, the same as the number of moles of solute in the resulting less concentrated solution.
Once you find your worksheet, click on popout icon or print icon to worksheet to print or. Easy speed math they dont teach you in school part 1 addition duration. Some of the worksheets displayed are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. Calculate the molarity of the resulting solution if a certain volume of water was added to 50. Topics you will need to know in order to pass the quiz. Serial dilutions austin community college district. Dilution worksheet and problems biology libretexts. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step in serial dilutions, you multiply the dilution factors for each step.
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